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Antibodies
Anti-HEC1/HEC antibody [9G3]
Anti-HEC1/HEC antibody [9G3] from Abcam
Be the first to write a review!
Citations:
(67)
Supplier Page
Supplier Page from
Abcam for
Anti-HEC1/HEC antibody [9G3]
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-HEC1/HEC antibody [9G3]
Company
Abcam
Price
Supplier Page
View Company Product Page
Catalog Number
ab3613
Antibody Type
Primary
Quantity
100 UL
Clonality
Monoclonal
Host
Mouse, Nude mouse
Isotype
IgG2a
Clone
9G3
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western blot
Conjugate/Tag
Unconjugated
Concentration
1 mg/ml
Gene Name
NDC80
Reactivity
Human, Pig
Target
HEC1
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (67)
(21)
Small-molecule inhibition of aging-associated chromosomal instability delays cellular senescence
EMBO reports
May 6, 2020
Barroso-Vilares M, Macedo JC, Reis M, Warren JD, Compton D, Logarinho E.
BD Biosciences
),
1:500
; rabbit anti‐cGAS (
15102
,
Cell Signaling Technology
),
1:200
;
mouse
anti‐Hec1
(
ab3613
,
Abcam
),
1:1,500
;
mouse
anti‐
Plk1
(
SC‐17783
,
Santa Cruz Biotechnology
),
1:2,000
; rabbit anti‐MCAK 49,
(22)
Ensemble-Level Organization of Human Kinetochores and Evidence for Distinct Tension and Attachment Sensors
Cell reports
April 28, 2020
Roscioli E, Germanova TE, Smith CA, Embacher PA, Erent M, Thompson AI, Burroughs NJ, McAinsh AD.
(
1:300
),
Enzo
,Cat#
ADI-KAM-CC006-E
; RRID:AB_2038993 "
Mouse
monoclonal
anti-Hec1
(9G3,
1:1000
)",
Abcam
,Cat#
ab3613
; RRID:AB_303949
Mouse
monoclonal anti-Bub1 (
1:200
),
Abcam
,Cat#
ab54893
; RRID:AB_940664
Mouse
monoclonal
(23)
Mis12 controls cyclin B1 stabilization via Cdc14B-mediated APC/C(Cdh1) regulation during meiotic G2/M transition in mouse oocytes
Development (Cambridge, England)
April 27, 2020
Bai GY, Choe MH, Kim JS, Oh JS.
(
Cell Signaling Technology
,
4138
;
1:1000
), anti-cyclin B2 (
R&D Systems
,
AF6004
;
1:1000
),
anti-Hec1
(
Abcam
,
ab3613
;
1:1000
), anti-Cdh1 (
Abcam
,
ab3242
;
1:1000
), anti-pY15-
Cdk1
(
Cell Signaling Technology
,
9111
;
1:1000
),
(24)
Endoplasmic reticulum stress participates in the pathophysiology of mercury-caused acute kidney injury
Renal Failure
November 19, 2019
Plácido Rojas-Franco, Margarita Franco-Colín, Alejandra Paola Torres-Manzo, Vanessa Blas-Valdivia, María del Rocio Thompson-Bonilla, Sinan Kandir, Edgar Cano-Europa
Cruz, CA:
sc-22799
,
sc-8327
,
sc-575
and
sc-7160
,
sc-30882
, respectively), and caspase-12 (
Millipore
,
AB3613
, Burlington, MA) diluted
1:500
, as well as IRE1α
Abcam
,
ab37073
) and ATF-4 (Biorbyt, orb-129518) diluted
(25)
Meiotic Kinetochores Fragment into Multiple Lobes upon Cohesin Loss in Aging Eggs
Current Biology
November 18, 2019
Agata P. Zielinska et al.
1:50
), rabbit anti-CENP-F (
ab5
,
Abcam
;
1:100
),
sheep
anti-BubR1
(
Abcam
,
ab28192
;
1:50
),
mouse
anti-Hec1
(
ab3613
,
Abcam
;
1:100
) and
rat
anti-α-tubulin (MCA78G, Serotec;
1:1000
). As secondary antibodies, Alexa Fluor488/564/647
(26)
Mammalian kinetochores count attached microtubules in a sensitive and switch-like manner
The Journal of cell biology
November 4, 2019
Kuhn J, Dumont S.
anti-
rat kangaroo
-Mad2 (Kuhn and Dumont, 2017; DeLuca et al., 2018;
1:100
),
mouse
anti-Hec1
(9G3;
1:100
,
ab3613
;
Abcam
), rabbit anti-mKate (recognizes FusionRed;
1:200
;
TA150072
;
OriGene
),
mouse
anti-hsMad1 (
1:300
;
(27)
Computational model demonstrates that Ndc80‐associated proteins strengthen kinetochore‐microtubule attachments in metaphase
Cytoskeleton (Hoboken)
November 1, 2019
Samuel Campbell, Mohammed Abdullahel Amin, Dileep Varma, Tamara Carla Bidone
figures or figure legends. The primary antibodies used in the study include Hec1 monoclonal (
1:400
,
ab3613
,
clone
9G3,
Abcam
),
Zwint1
polyclonal (
1:400
,
A300-781A
,
Bethyl
), Tubulin monoclonal (
1:500
,
T9026
,
(28)
Spindle checkpoint silencing at kinetochores with submaximal microtubule occupancy
Journal of Cell Science
March 5, 2019
Banafsheh Etemad, Abel Vertesy, Timo E. F. Kuijt, Carlos Sacristan, Alexander van Oudenaarden, Geert J. P. L. Kops
(A11031). The following primary antibodies were used
for immunoblotting
: HEC1 (
mouse
monoclonal,
1:1000
;
Abcam
,
ab3613
), α-tubulin (
mouse
monoclonal,
1:10
.000;
Sigma
,
T5168
), mCherry (rabbit polyclonal,
1:1000
;
Abcam
,
ab167453
),
(29)
The dynein adaptor Hook2 plays essential roles in mitotic progression and cytokinesis
The Journal of cell biology
March 4, 2019
Dwivedi D, Kumari A, Rathi S, Mylavarapu SVS, Sharma M.
protein/
CYK4
(
A302-797A
;
Bethyl Laboratories
), rabbit
anti-Arp1
(
ab11009
;
Abcam
),
mouse
anti-Hec1/Ndc80 (9G3;
ab3613
;
Abcam
), rabbit anti-Cyclin-B (Y106;
ab32053
;
Abcam
), rabbit anti-CENP-F (
ab5
;
Abcam
), rabbit
anti-p50/dynamitin
(30)
LUBAC controls chromosome alignment by targeting CENP-E to attached kinetochores
Nature communications
January 17, 2019
Wu M, Chang Y, Hu H, Mu R, Zhang Y, Qin X, Duan X, Li W, Tu H, Zhang W,
et al
.
from Dr. Iain M. Cheeseman), rabbit anti-Aurora B (
1:200
,
A300-431A
,
BETHYL
),
mouse
anti-Hec1
(
1:200
,
ab3613
,
Abcam
), rabbit anti-Spc25 (
1:200
, S1215, Epitomics), rabbit
anti-MCAK
(
1:200
, AKIN05,
cytoskeleton
),
(31)
NudCL2 is an Hsp90 cochaperone to regulate sister chromatid cohesion by stabilizing cohesin subunits
Cellular and molecular life sciences : CMLS
January 1, 2019
Yang Y, Wang W, Li M, Gao Y, Zhang W, Huang Y, Zhuo W, Yan X, Liu W, Wang F,
et al
.
T6199
), cyclin B1 (
Santa Cruz
,
sc-70898
), Bub1 (Abcam, ab900), Mad2 (
Santa Cruz
,
sc-28261
), Hec1 (
Abcam
,
ab3613
) and
SMC2
(
Abcam
,
ab10412
) and
human
CREST autoimmune serum (
Antibodies, Inc
.,
15-235
-0001) were used.
(32)
Self-Assembly of the RZZ Complex into Filaments Drives Kinetochore Expansion in the Absence of Microtubule Attachment
Current biology : CB
November 5, 2018
Pereira C, Reis RM, Gama JB, Celestino R, Cheerambathur DK, Carvalho AX, Gassmann R.
anti-GFP (
clone
9F9.F9),
Abcam
,Cat No.:
ab1218
; RRID: AB_298911
Mouse
anti-HEC1
(
clone
9G3),
Abcam
,Cat No.:
ab3613
: RRID: AB_303949
Mouse
anti-MAD1
(
clone
BB3-8),
Millipore
,Cat No.:
MABE867
/6B7115 Mouse anti-MAD2,
Santa Cruz Biotechnology
(33)
Chromosome Segregation Is Biased by Kinetochore Size
Current biology : CB
May 7, 2018
Drpic D, Almeida AC, Aguiar P, Renda F, Damas J, Lewin HA, Larkin DM, Khodjakov A, Maiato H.
B512),
Sigma-Aldrich
,Cat#
T5168
Mouse
anti - CENP-A
,[22],Lars E.T. Jansen Rabbit anti - Hec1,
Abcam
,Cat#
ab3613
Mouse
anti - cMad2,
Santa Cruz
,Cat#
sc-65492
Rabbit anti - Mad2:,
Bethyl Laboratories
,Cat#
A300-301A
"Rabbit
(34)
Disentangling the molecular determinants for Cenp-F localization to nuclear pores and kinetochores
EMBO reports
May 1, 2018
Berto A, Yu J, Morchoisne-Bolhy S, Bertipaglia C, Vallee R, Dumont J, Ochsenbein F, Guerois R, Doye V.
I. Mattaj, EMBL, Heidelberg, Germany;
1:1,000
; [63]);
mouse
monoclonal
anti-HEC1
(
clone
9G3;
Abcam
ab3613
;
1:500
); rabbit antiCenp-E antibody (generously provided by T.J. Yen, Philadelphia, USA;
1:1,000
; [22]);
(35)
Seh1 targets GATOR2 and Nup153 to mitotic chromosomes
Journal of cell science
May 1, 2018
Platani M, Samejima I, Samejima K, Kanemaki MT, Earnshaw WC.
(rabbit; cat. no.
AB21582
:
Abcam
); SKAP,
1:300
(Schmidt et al., 2010); Hec1,
1:300
(
mouse
; cat. no.
ab3613
,
Abcam
); Nup153,
1:200
(
mouse
; cat. no.
ab24700
,
Abcam
); CenpC,
1:100
(
mouse
; cat. no.
ab50974
,
Abcam
);
(36)
Aurora B prevents premature removal of spindle assembly checkpoint proteins from the kinetochore: A key role for Aurora B in mitosis
Oncotarget
April 13, 2018
Gurden MD, Anderhub SJ, Faisal A, Linardopoulos S.
(
Millipore
,
05–682
),
MPS1
pT33pS37 (
Life Technologies
,
44–1325G
),
GFP
(
Abcam
,
ab6556
), HEC1 (
Abcam
,
ab3613
),
Histone H3
pS10 (
Millipore
,
06–570
),
KNL1
(
Bethyl Laboratories Inc
.,
A300-805A
), SPINDLY (Abnova,
(37)
HP1α targets the chromosomal passenger complex for activation at heterochromatin before mitotic entry
The EMBO journal
March 15, 2018
Ruppert JG, Samejima K, Platani M, Molina O, Kimura H, Jeyaprakash AA, Ohta S, Earnshaw WC.
Santa Cruz Biotechnology
), anti‐Dsn1ph (
1:1,000
; a kind gift of Iain Cheeseman; Welburn et al, 2010),
anti‐Hec1
(9G3
ab3613
;
1:500
;
Abcam
). Cells grown on coverslips were fixed in a pre‐warmed 4% formaldehyde/PBS solution for
(38)
Mitosis-specific acetylation tunes Ran effector binding for chromosome segregation
Journal of Molecular Cell Biology
February 1, 2018
Xiaoling Bao et al.
(
Sigma-Aldrich
). In
immunofluorescence assay
, the following antibodies were used:
mouse
anti-Hec1
(
ab3613
,
Abcam
) and rabbit anti-tubulin (2871-1, Epitomics). Secondary antibodies were purchased from Jackson
(39)
ROS-induced near-homozygous genomes in thyroid cancer
Endocrine-Related Cancer
January 1, 2018
Willem E Corver, Joris Demmers, Jan Oosting, Shima Sahraeian, Arnoud Boot, Dina Ruano, Tom van Wezel, Hans Morreau
(
blotting
) (mouse IgG1, Cat. No.
T9026
,
Sigma-Aldrich
),
anti-HEC1
,
clone
9G3,
1:1000
(mouse IgG2a, Cat. No.
ab3613
,
Abcam
), antipCHK2 (Thr68) (rabbit IgG, Cat. No.
2197
,
Cell Signaling Technology
). Secondary reagents:
(40)
DISC1 Regulates Neurogenesis via Modulating Kinetochore Attachment of Ndel1/Nde1 during Mitosis
Neuron
December 6, 2017
Fei Ye et al.
images were acquired with a Leica sp8
confocal
microcopy.
Mouse
Hec1 primary antibody is from
Abcam
(
ab3613
). Rabbit Ndel1 primary antibody was provided by Shinji Hirotsune at Osaka City University (Sasaki et
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Anti-HEC1/HEC antibody [9G3] from Abcam
Supplier Page
View Company Product Page
Product Specs
Company Info
Citations
Reviews
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